Figure 5

Electrophoretic pattern of nested RT –PCR and phylogenetic tree of HCV RNA positive individuals. A: Agarose gel picture of nested RT- PCR amplified product of 5'NCR of HCV genome. Lane 1: MW (100 bp DNA ladder); lane 2: positive control; lane 3: negative control; lane 3, 4, 5, 7, 8, 9, 10, 11, 12, 13 depicts the positive sample numbered HIV35, HIV61, HIV88, HIV97, HIV153, HIV173, HIV 175, HIV 176, HIV177 and HIV 190 respectively. B: Phylogenetic analysis of hepatitis C virus 5' non coding region. Phylogenetic analysis of 5' non coding region (nt -240 to -60; 181 bp) sequences of 10 HCV samples of HIV seropositive individuals. The sequences for major subtype were selected from the GeneBank database for analysis. The accession Numbers of the reference sequences (with subtypes are as follows: AF011753 (1A), AJ132996 (1B), AY051292 (1C), af238485 (2a), af238486 (2b), L38330 (2c), AF046866 (3a), D49374 (3b), D16612 (3c), D16620 (3d), D16618 (3e), X91421 (3g), Y11604 (4a), Y13184 (5a), Y12083 (6a) and the samples are numbered as HIV following the reference number.