Figure 1

Antiviral effect of siRNAs against HCV viral replication. Huh-7 cells were infected with high titer sera sample from HCV-1a patients to establish in vitro cell culture model of HCV-1a, cells were maintained overnight at 37°C in 5% CO2 for three days. Cells were harvested after siRNA treatment 48 hrs post transfection and intracellular HCV RNA levels were quantified by Real Time PCR. Data is expressed as percentage of HCV survival in cells. Error bars indicate, mean S.D p < 0.05 verses control.