Figure 2

Vaccine elicited anti-DIII antibodies. Total IgG titers were measured by ELISA on WNV DIII-coated plates from mice vaccinated ID or IM with DNA plasmids encoding sections of the WNV E gene, with or without molecular adjutant P28 on week 8. Each dot represents an individual mouse. Undetectable antibody titers were arbitrarily assigned a titer of 1. Error bars denote the standard error within the samples with a measurable titer. Representative data from 1 of 2 experiments shown. A 2-way unmatched ANOVA with a Bonferroni post-test was used to determine the significance of the data between groups, which is denoted by asterisks; * P < 0.05, ** P < 0.01, *** P < 0.001.