Figure 3
From: Human herpesvirus 6 infection impairs Toll-like receptor signaling
Impairment of TLR4 signaling in HHV-6-infected DCs. (A) Western blotting reveals that the expression level of TLR4 protein in HHV-6-infected DCs, which were not stimulated with LPS, is slightly higher than that in mock-infected DCs. (B) Flow cytometric analysis using fluorescent LPS conjugate reveals that the amount of LPS bound to HHV-6-infected DCs is slightly higher than that on mock-infected DCs, suggesting that the expression level of TLR4 molecules on DCs is increased after infection with HHV-6. (C) Western blotting reveals that the expression levels of MyD88 and TRAF6 proteins in HHV-6-infected DCs, which are not stimulated with LPS, are slightly higher than those in mock-infected DCs. (D) Western blotting reveals that phosphorylation levels of TAK-1, IKKα/β and IκB-α in HHV-6-infected DCs after stimulation with LPS are significantly lower than those in LPS-stimulated mock-infected DCs.