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Table 2 Determination of optimized primers concentration for the PRRSV one step real-time RT-PCR

From: The development of a rapid SYBR one step real-time RT-PCR for detection of porcine reproductive and respiratory syndrome virus

primers concentration (μM)

Cycle threshold (Ct)

0.1

> 40

0.2

37.11

0.3

29.05

0.4

25.60

0.5

25.21

0.6

24.90

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Virology Journal

ISSN: 1743-422X