Skip to main content

Table 1 Annealing temperature of Nf/Nr oligonucleotide pair optimization by means of Ct values

From: The development of a rapid SYBR one step real-time RT-PCR for detection of porcine reproductive and respiratory syndrome virus

Annealing temperature

Cycle threshold (Ct)

53

23.12

55

22.90

56

21.26

57

23.78

58

25.62

Back to article page

Virology Journal

ISSN: 1743-422X