Figure 4From: The development of a rapid SYBR one step real-time RT-PCR for detection of porcine reproductive and respiratory syndrome virusThe sensitivity of real-time qRT-PCR for detection of the PRRSV. A 10-fold dilution series of total RNA extracted from a field sample ranging from 10-1 to 10-7 dilutions were tested in parallel in the qRT-PCR assay and in the conventional RT-PCR. The detection limit for the real-time qRT-PCR assay was a 10-5 dilution of sample RNA.Back to article page