Figure 6

Fusion of SIV resistant and susceptible rhesus monkey B-LCLs demonstrates a dominant SIV resistance phenotype. SIV susceptible and resistant B-LCLs were labeled with either Vybrant DID (Invitrogen) or Oregon Green (Invitrogen). Susceptible and resistant cell lines were fused using PEG incubation and double positive fusion events were sorted by flow cytometry. Fused cells were subsequently infected with SIVsmE543-GFP and quantified for % GFP+ by flow cytometry.