Figure 2

Rabbit anti-FV3 serum neutralizes an FV3 infection in a mammalian cell line. Rabbit anti-FV3 serum (0-100 ng) or rabbit pre-immune serum (0-100 ng) was incubated with FV3 (~50 PFU) before being added to BGMK or BF-2 cells for 2 hours. Once infected, BGMK cells were incubated at 28°C with 5% CO₂. The cells were subsequently overlaid with agarose. (A) Forty-eight hours post-overlay BGMK cells underwent indirect immunofluorescence and plaques were counted. Plaque numbers from 3 independent trials were counted and mean plaque values were compared to BGMK cells infected with FV3 only and values are shown as a relative percentage. (B) Forty-eight hours post-overlay, BGMK and BF-2 cells were processed for indirect immunofluorescence using rabbit anti-FV3 serum (green) and DAPI (nuclei - blue).