Comparison of transgene expression in A549 cells infected with Ad5 monovalent or trivalent formulations. A549 cells were infected as described in figure 1 with monovalent or trivalent Ad5 vectors. Gag, pol and nef proteins were detected by colorimetric ELISA (A), gag, pol and nef mRNAs were detected by RT-qPCR (B). The housekeeping gene GAPDH mRNA was monitored to address cell alteration (B). The MOI used at infection were normalized for transgene concentration, not for total virus concentration. The error bars represent 95% confidence interval.