Table 3 Relative quantification methods of differentially expressed genes two days post inoculation.
Genes | Identity | Relative quantification against Unit mass | Relative quantification Normalized to a reference gene | |||
|---|---|---|---|---|---|---|
Control | Healthy | Livak method | ΔCT Method | Pfaffi Method | ||
SAA | S. lycopersicum WRKY transcription factor IId-1 splice | 2.32 | 1.32 | 0.737C/0.381H | 0.942C/0.838H | 0.737C/0.381H |
SAB | Putative Rieske iron-sulfur protein [A. thaliana]Length = 539, Rieske iron-sulfur protein Tic55 [P. sativum]Length = 553 | 2.751 | 0.566 | 0.870C/0.162H | 0.972C/0.728H | 0.870C/0.162H |
SAC | N. tabacum mitochondrial DNA, complete genome Length = 430597 NADH dehydrogenase subunit 1 NADH dehydrogenase subunit 2, 846 bp at 5' side | 0.010 | 1.905 | 0.003C/0.547H | -0.672C/1.355H | 0.003C/0.547H |
SAD | Quinonprotein alcohol dehydrogenase like [M. truncatula] | 5.205 | 0.829 | 1.647C/0.238H | 1.167C/0.707H | 1.647C/0.238H |