Figure 4

The effect of ChCypA on avian influenza virus infectivity. A. CEF cells were infected with chCypA-expressing recombinant adenovirus rAdchCypA (or rAd as control) and infected after 30 h with A/Chicken/Liaoning/1/00 (H9N2) (MOI = 1), 4 h post influenza virus infection, cells were stained for NP (red). The percentage of influenza virus antigen positive cells in GFP positive population relative to that for the rAd control group was shown below. Scale bar: 20 μm. *P < 0.05. B. ChCypA inhibited expression of M1 protein. 293T/CypA- cells were transfected with pCMV-Myc or pCMV-Myc-chCypA, 24 h post transfection, cells were infected with A/Chicken/Liaoning/1/00 (H9N2) (MOI = 0.01). 6 h and 9 h post infection, cells were harvested. Proteins were detected by Western blot analysis using anti-β-actin, M1 and c-Myc monoclonal antibodies as indicated. The relative expression level of M1 protein was indicated by densitometry of M1 band on Western blot X film. The densitometry of target protein band was analyzed with Photoshop program.