Figure 3

The effect of chCypA on A/WSN/33 virus production. The inhibitory effect of chCypA on influenza virus replication was tested by using reverse genetic system of A/WSN/33 [49]. In brief, both a mixture of expression plasmids of PB1, PB2, PA and NP proteins and a whole set of virus RNA expression plasmids were transfected into 293T/CypA- cells in the presence or absence of chCypA expression plasmid pCMV-Myc-chCypA, pCMV-Myc was transfected as control. The supernatant was harvested 48 hpt and titrated on MDCK cells using plaque assay. Significant differences across control were indicated with two asterisks at P < 0.01.