Figure 1
From: Replication and transcription of human papillomavirus type 58 genome in Saccharomyces cerevisiae
Replication of HPV 58 genome in S. cerevisiae. A and B: Episomal replication of HPV58 genome in S. cerevisiae. Yeast DNA underwent Southern blot with an L1 specific mRNA probe. A, (DNA isolated from yeast harboring HPV58-Ura) Lane 1: yeast DNA without enzyme digestion; lane 2-5: yeast DNA digested with Dpn I, Xho I, Bgl II and Hpa I. B, Lane 1: DNA isolated from HPV58-Ura-E2mt transformed yeast and treated with Dpn I; lanes2-6 (yeast DNA isolated from HPV58-Ura-E2mt/pDBLeu-E2 transformed yeast), lane 2: DNA without enzyme digestion; lanes 3-6: yeast DNA digested with Dpn I, Xho I, Bgl II and Hpa I separately. Arrows show the positions of open-circle (OC), linear (L) and supercoiled (SC) forms of episomal HPV58-Ura or HPV58-Ura-E2mt. C. qRT-PCR to compare the E2 transcription levels in yeast cells harboring HPV58-Ura or pDBLeu-E2. D. Expression of E2 protein in yeast. Yeast protein was prepared from yeast harboring pDBLeu-E2 (lane 1), pDBLeu (lane 2), and untransformed yeast (lane 3).