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Figure 6 | Virology Journal

Figure 6

From: Protection against H1N1 influenza challenge by a DNA vaccine expressing H3/H1 subtype hemagglutinin combined with MHC class II-restricted epitopes

Figure 6

Humoral immune responses in vaccinated mice. ELISAs to detect virus-specific IgG antibodies were performed utilizing plates coated with (A) A/Wisconsin/67/2005 (H3N2), (B) A/New Caledonia/20/99 (H1N1), or (C) influenza standard antigens of H5, H7, H9 subtypes. Data shown are mean antibody titers of five mice in each group with coefficients of variations (error bars). The differences in titers between experimental groups and the negative control group (pVAX1) were statistically significant (P < 0.01). However, the differences in the responses against the H3N2 and H1N1 antigens in the ELISA between the multi-epitope pV-H3-EHA-H1 and pV-H3 and between pV-H1 and pV-H3-H1 groups were not significant (P > 0.05). With the H5, H7 and H9 standard antigens as the coated proteins in the ELISA, the responses in the pV-H3-EHA-H1 group were significantly higher than those of the other vaccine groups at 35 DPI (P < 0.05).

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Virology Journal

ISSN: 1743-422X