Figure 7

Antiviral effect IFN-α on the replication of JFH1-GFP RNA using S-Huh-7 and R-Huh-7 cells in culture. (A) S-Huh-7 and R-Huh-7 cells were transfected with full-length JFH1-GFP RNA and next day the cultures were treated with IFN-α (1000 IU/ml). Intracellular GFP expression was measured after 24, 48, 72 and 96 hours of post-transfection. Upper panel shows the intracellular GFP expression after IFN-α treatment in S-Huh-7 cells. Bottom panel shows the intracellular GFP expression in R-Huh-7 cells after IFN-α treatment (B) Quantification of antiviral effect of IFN-α (1000 IU/ml) between these two cell lines by flow analysis after 72 hours of post-transfection. In the S-Huh-7 cell line the number of GFP positive cells were decreased from 4.2% to 0.2% after IFN-α treatment. There is no decrease in the number of GFP positive cells after IFN-α treatment in the transfected R-Huh-7 cells. The upper and lower left panel shows the untransfected cells as mock. (C) Intracellular HCV mRNA levels in the IFN treated cells measured by RPA. The experiments were carried out same way as described in the panel A; except that the RPA analysis was performed using the total RNA isolated from the transfected cells at different time points. Upper panel shows the HCV RNA level in the transfected S-Huh-7 cells after IFN-α treatment. Bottom panel shows the HCV RNA levels in the transfected R-Huh-7 cells after IFN-α treatment.