Figure 6
From: Characterization of viroplasm formation during the early stages of rotavirus infection
Viroplasms do not colocalize with Cy5 labeled infectious rotavirus particles. MA104 cells grown in coverslips were infected with purified rotavirus strain RRV TLPs (left side panels) or purified rotavirus strain RRV TLP's labeled with Cy5 (right side panels). Four hours after infection, cells were fixed, and the viroplasms detected with an anti-NSP2 polyclonal antibody, and a secondary antibody coupled to Alexa-488. Images were obtained with confocal microscope LSM 510 and processed as described in material and methods. In merge, detected viroplasms are in green, and Cy5 labeled RRV particles are in red, pointed by arrows. Detail of viroplasm location and Cy5 labeled RRV are shown.