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Figure 1 | Virology Journal

Figure 1

From: Targeting lentiviral vector to specific cell types through surface displayed single chain antibody and fusogenic molecule

Figure 1

Schematic representation of key constructs in this study. These constructs include a lentiviral backbone vector FUGW, fusogenic molecule (FM) derived from Sindbis virus glycoprotein, membrane-bound single chain antibody against the CD20 antigen with either a HLA-A2 transmembrane domain (SC2H7-A2) or a VSVG transmembrane domain (SC2H7-GS). CMV enhancer: the enhancer element derived from human cytomegalovirus; GFP: enhanced green fluorescence protein; Ubi: the human ubiquitin-C promoter; WRE: woodchuck responsive element; CMV: human cytomegalovirus immediate-early gene promoter; αCD20 Vκ: the variable domain of the kappa chain of the mouse anti-CD20 antibody; αCD20 Vγ: the variable domain of the gamma chain of the mouse anti-CD20 antibody; CH2-CH3 region: the CH2-CH3 region of the human IgG1 antibody; HLA-A2 transmembrane domain: the transmembrane domain of the HLA-A2 protein; VSVG transmembrane domain: the transmembrane domain of the VSVG protein; E3: the leading peptide of Sindbis virus glycoprotein; E1: the E1 protein of Sindbis virus glycoprotein for mediating fusion; E2: the E2 protein of Sindbis virus glycoprotein for binding to cellular receptor; HA tag: 10-amino acid epitope sequence of hemagglutim; pA: polyadenylation signal.

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Virology Journal

ISSN: 1743-422X