Figure 2

Meq inhibits the transcriptional activity of p53. (A and B) H1299 cells were co-transfected transiently with a combination of the indicated plasmids in the presence of the p53 luciferase reporter plasmid (p53-Luc). The expression of the indicated plasmids was detected by western blot analysis (A). The luciferase activity of the transfectants was measured 24 h post-transfection (B). *p < 0.05 compared with cells transfected with Flag-p53 alone (Flag-p53+Flag-Vec). (C) H1299 cells were transfected transiently with increasing amounts of Flag-Meq in the presence of Flag-p53 and p53-Luc. The expression of Flag-Meq and Flag-p53 was detected by western blot analysis. *p < 0.05 compared with cells transfected with Flag-p53 alone (Flag-p53+Flag-Vec). (D and E) CEF cells were transfected transiently with the indicated plasmids in the presence of p21 promoter luciferase reporter plasmids (D) or MDM2 promoter luciferase reporter plasmids (E). *p < 0.05 compared with cells transfected with Flag-vector (Flag-Vec).