Figure 1

Characterization of recombinant scFv antibodies. A) Immunoblot analysis of recombinant scFvs. Concentrated 76-118 and L99 virus were separated on 12% SDS-PAGE and blotted on PVDF membrane, parallel treated normal vero E6 cells culture supernatant was used as negative control, the membrane was incubated with prokaryotic produced and purified scFv, and mouse anti- penta-His tag monoclonal antibody, detected with horseradish peroxidase-conjugated goat anti-mouse antibody. B) Immunofluorescence analysis of recombinant scFvs, recombinant N protein expressing SF9 cells were fixed on dot slides, incubated with scFvs, (1) 76-118 N incubate with L13F scFv; (2) L99 N incubate with L13F scFv; (3) 76-118 N incubate with H34 scFv; (4) L99 N incubate with H34 scFv. C) Intracellular localization of scFvs. COS-7 cells were seeded onto a 6-well plate, grown to 80% confluency, and transfected with intracellular antibodies expressing plasmid (1) L13F3-scFv-CYTO; (2) H34-scFv-CYTO; (3) L13F3-scFv-ER and (4) H34-scFv-ER. The cells were fixed at 48 h posttransfection, and the scFvs was identified with IFA by using anti-His MAb, images were obtained with confocal microscope with 100 × objectives.