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Table 1 Assay Reproducibility (HSV-2)

From: Genital herpes evaluation by quantitative TaqMan PCR: correlating single detection and quantity of HSV-2 DNA in cervicovaginal lavage fluids with cross-sectional and longitudinal clinical data

    

Intra-assay

Inter-assay

# copies per assay

Run

Ct1

Ct2

mean Ct

Ct SD

CV (Ct)

mean Ct

Ct SD

CV (Ct)

150,000

1

24.11

23.99

24.05

0.08

0.34

24.05

0.11

0.46

 

2

24.18

24.13

24.15

0.04

0.15

   
 

3

24.12

23.74

23.93

0.27

1.13

   

15,000

1

27.34

27.28

27.31

0.04

0.14

27.25

0.16

0.57

 

2

27.46

27.25

27.36

0.15

0.55

   
 

3

26.97

27.16

27.07

0.13

0.50

   

1,500

1

30.67

30.56

30.62

0.08

0.25

30.76

0.14

0.45

 

2

30.97

30.81

30.89

0.12

0.38

   
 

3

30.81

30.74

30.77

0.05

0.16

   

150

1

34.82

33.90

34.36

0.66

1.91

34.30

0.25

0.74

 

2

34.50

34.52

34.51

0.01

0.03

   
 

3

34.09

33.94

34.02

0.10

0.31

   

15

1

37.09

37.44

37.27

0.25

0.67

37.41

0.15

0.41

 

2

37.83

37.32

37.57

0.36

0.96

   
 

3

38.50

36.25

37.38

1.59

4.26

   
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Virology Journal

ISSN: 1743-422X