Prediction of conformational changes by single mutation in the hepatitis B virus surface antigen (HBsAg) identified in HBsAg-negative blood donors

Ie, Susan I; Thedja, Meta D; Roni, Martono; Muljono, David H

doi:10.1186/1743-422X-7-326

Virology Journal

Table 1 The Jameson-Wolf antigenicity index prediction of HBsAg within amino acid 118 - 160

From: Prediction of conformational changes by single mutation in the hepatitis B virus surface antigen (HBsAg) identified in HBsAg-negative blood donors

Position	M54923		Pattern T123A*		Pattern T123N**		Pattern M133L*		Pattern M133I**		Pattern M133T**		Pattern M133V**		Pattern T143M*		Pattern T143L**
	Residue	Antigenic Index	Residue	Antigenic Index	Residue	Antigenic Index	Residue	Antigenic Index	Residue	Antigenic Index	Residue	Antigenic Index	Residue	Antigenic Index	Residue	Antigenic Index	Residue	Antigenic Index
110	Ile	-0.2	Ile	-0.2	Ile	-0.2	Ile	-0.2	Ile	-0.2	Ile	-0.2	Ile	-0.2	Ile	-0.2	Ile	-0.2
111	Pro	-0.05	Pro	-0.05	Pro	-0.05	Pro	-0.05	Pro	-0.05	Pro	-0.05	Pro	-0.05	Pro	-0.05	Pro	-0.05
112	Gly	0.35	Gly	0.35	Gly	0.35	Gly	0.35	Gly	0.35	Gly	0.35	Gly	0.35	Gly	0.35	Gly	0.35
113	Ser	0.8	Ser	0.8	Ser	0.8	Ser	0.8	Ser	0.8	Ser	0.8	Ser	0.8	Ser	0.8	Ser	0.8
114	Ser	1	Ser	1	Ser	1	Ser	1	Ser	1	Ser	1	Ser	1	Ser	1	Ser	1
115	Thr	0.4	Thr	0.4	Thr	0.4	Thr	0.4	Thr	0.4	Thr	0.4	Thr	0.4	Thr	0.4	Thr	0.4
116	Thr	1.05	Thr	1.05	Thr	*0.8*	Thr	1.05	Thr	1.05	Thr	1.05	Thr	1.05	Thr	1.05	Thr	1.05
117	Ser	1.3	Ser	1.3	Ser	*1.05*	Ser	1.3	Ser	1.3	Ser	1.3	Ser	1.3	Ser	1.3	Ser	1.3
118	Thr	1.2	Thr	1.2	Thr	*0.95*	Thr	1.2	Thr	1.2	Thr	1.2	Thr	1.2	Thr	1.2	Thr	1.2
119	Gly	2.05	Gly	2.05	Gly	*1.6*	Gly	2.05	Gly	2.05	Gly	2.05	Gly	2.05	Gly	2.05	Gly	2.05
120	Pro	2.5	Pro	2.5	Pro	*2.05*	Pro	2.5	Pro	2.5	Pro	2.5	Pro	2.5	Pro	2.5	Pro	2.5
121	Cys	2.25	Cys	2.25	Cys	*2.5*	Cys	2.25	Cys	2.25	Cys	2.25	Cys	2.25	Cys	2.25	Cys	2.25
122	Lys	2	Lys	*1.6*	Lys	*2.25*	Lys	2	Lys	2	Lys	2	Lys	2	Lys	2	Lys	2
123	Thr	0.35	Ala	*0.4*	Asn	2	Thr	0.35	Thr	0.35	Thr	0.35	Thr	0.35	Thr	0.35	Thr	0.35
124	Cys	0.25	Cys	*0.15*	Cys	*1.5*	Cys	0.25	Cys	0.25	Cys	0.25	Cys	0.25	Cys	0.25	Cys	0.25
125	Thr	0.45	Thr	*0.65*	Thr	*0.9*	Thr	0.45	Thr	0.45	Thr	0.45	Thr	0.45	Thr	0.45	Thr	0.45
126	Thr	0.25	Thr	0.25	Thr	0.25	Thr	0.25	Thr	0.25	Thr	0.25	Thr	0.25	Thr	0.25	Thr	0.25
127	Pro	0.4	Pro	0.4	Pro	0.4	Pro	0.4	Pro	0.4	Pro	0.4	Pro	0.4	Pro	0.4	Pro	0.4
128	Ala	0.8	Ala	0.8	Ala	0.8	Ala	0.8	Ala	0.8	Ala	0.8	Ala	0.8	Ala	0.8	Ala	0.8
129	Gln	0.8	Gln	0.8	Gln	*0.4*	Gln	0.8	Gln	0.8	Gln	0.8	Gln	0.8	Gln	0.8	Gln	0.8
130	Gly	0.65	Gly	0.65	Gly	0.65	Gly	0.65	Gly	*0.25*	Gly	*0.8*	Gly	*0.25*	Gly	0.65	Gly	0.65
131	Thr	0.35	Thr	0.35	Thr	0.35	Thr	0.35	Thr	*-0.05*	Thr	0.35	Thr	*-0.05*	Thr	0.35	Thr	0.35
132	Ser	0.35	Ser	0.35	Ser	0.35	Ser	0.35	Ser	*-0.05*	Ser	*0.65*	Ser	*-0.05*	Ser	0.35	Ser	0.35
133	Met	-0.2	Met	-0.2	Met	-0.2	Leu	*-0.05*	Ile	*-0.6*	Thr	*0.8*	Val	*-0.45*	Met	-0.2	Met	-0.2
134	Phe	-0.2	Phe	-0.2	Phe	-0.2	Phe	-0.2	Phe	-0.2	Phe	*-0.05*	Phe	-0.2	Phe	-0.2	Phe	-0.2
135	Pro	0.2	Pro	0.2	Pro	0.2	Pro	0.2	Pro	0.2	Pro	*0.35*	Pro	0.2	Pro	0.2	Pro	0.2
136	Ser	0.2	Ser	0.2	Ser	0.2	Ser	0.2	Ser	0.2	Ser	0.2	Ser	0.2	Ser	0.2	Ser	0.2
137	Cys	0.2	Cys	0.2	Cys	0.2	Cys	0.2	Cys	0.2	Cys	0.2	Cys	0.2	Cys	0.2	Cys	0.2
138	Cys	0.64	Cys	0.64	Cys	0.64	Cys	0.64	Cys	0.64	Cys	0.64	Cys	0.64	Cys	*0.3*	Cys	*0.3*
139	Cys	1.18	Cys	1.18	Cys	1.18	Cys	1.18	Cys	1.18	Cys	1.18	Cys	1.18	Cys	*0.5*	Cys	*0.8*
140	Thr	1.27	Thr	1.27	Thr	1.27	Thr	1.27	Thr	*1.67*	Thr	1.27	Thr	1.27	Thr	*0.41*	Thr	*0.7*
141	Lys	2.36	Lys	2.36	Lys	2.36	Lys	2.36	Lys	2.36	Lys	2.36	Lys	2.36	Lys	*1.47*	Lys	*1.75*
142	Pro	3.4	Pro	3.4	Pro	3.4	Pro	3.4	Pro	3.4	Pro	3.4	Pro	3.4	Pro	*2.33*	Pro	*2.6*
143	Thr	2.86	Thr	2.86	Thr	2.86	Thr	2.86	Thr	2.86	Thr	2.86	Thr	2.86	Met	*2.74*	Leu	3
144	Asp	2.57	Asp	2.57	Asp	2.57	Asp	2.57	Asp	2.57	Asp	2.57	Asp	2.57	Asp	*3.1*	Asp	*2.45*
145	Gly	1.93	Gly	1.93	Gly	1.93	Gly	1.93	Gly	1.93	Gly	1.93	Gly	1.93	Gly	*2.49*	Gly	*2.15*
146	Asn	1.59	Asn	1.59	Asn	1.59	Asn	1.59	Asn	1.59	Asn	1.59	Asn	1.59	Asn	*1.58*	Asn	*1.25*
147	Cys	0.1	Cys	0.1	Cys	0.1	Cys	0.1	Cys	0.1	Cys	0.1	Cys	0.1	Cys	*0.72*	Cys	*0.4*
148	Thr	-0.6	Thr	-0.6	Thr	-0.6	Thr	-0.6	Thr	-0.6	Thr	-0.6	Thr	-0.6	Thr	*-0.29*	Thr	-0.6
149	Cys	-0.6	Cys	-0.6	Cys	-0.6	Cys	-0.6	Cys	-0.6	Cys	-0.6	Cys	-0.6	Cys	-0.6	Cys	-0.6
150	Ile	-0.6	Ile	-0.6	Ile	-0.6	Ile	-0.6	Ile	-0.6	Ile	-0.6	Ile	-0.6	Ile	-0.6	Ile	-0.6
151	Pro	-0.6	Pro	-0.6	Pro	-0.6	Pro	-0.6	Pro	-0.6	Pro	-0.6	Pro	-0.6	Pro	-0.6	Pro	-0.6
152	Ile	-0.45	Ile	-0.45	Ile	-0.45	Ile	-0.45	Ile	-0.45	Ile	-0.45	Ile	-0.45	Ile	-0.45	Ile	-0.45
153	Pro	-0.05	Pro	-0.05	Pro	-0.05	Pro	-0.05	Pro	-0.05	Pro	-0.05	Pro	-0.05	Pro	-0.05	Pro	-0.05
154	Ser	0.35	Ser	0.35	Ser	0.35	Ser	0.35	Ser	0.35	Ser	0.35	Ser	0.35	Ser	0.35	Ser	0.35
155	Ser	0.35	Ser	0.35	Ser	0.35	Ser	0.35	Ser	0.35	Ser	0.35	Ser	0.35	Ser	0.35	Ser	0.35
156	Trp	-0.2	Trp	-0.2	Trp	-0.2	Trp	-0.2	Trp	-0.2	Trp	-0.2	Trp	-0.2	Trp	-0.2	Trp	-0.2
157	Ala	-0.6	Ala	-0.6	Ala	-0.6	Ala	-0.6	Ala	-0.6	Ala	-0.6	Ala	-0.6	Ala	-0.6	Ala	-0.6
158	Phe	-0.6	Phe	-0.6	Phe	-0.6	Phe	-0.6	Phe	-0.6	Phe	-0.6	Phe	-0.6	Phe	-0.6	Phe	-0.6
159	Ala	-0.6	Ala	-0.6	Ala	-0.6	Ala	-0.6	Ala	-0.6	Ala	-0.6	Ala	-0.6	Ala	-0.6	Ala	-0.6
160	Lys	-0.6	Lys	-0.6	Lys	-0.6	Lys	-0.6	Lys	-0.6	Lys	-0.6	Lys	-0.6	Lys	-0.6	Lys	-0.6

*Variants found in Indonesian blood donor; **Variants frequently associated with problems in diagnostic assays and/or escape to vaccine/HBIg therapy. Residues with substitutions and their positions are shown in bold. Altered antigenicity index of affected residues in each substitution pattern are shown in bold and italics: T123A alters four consecutive residues (aa 122-125); M133L alters the antigenic index of position 133 only; T123N, M133I/T/V, and T143L cause relatively extensive antigenic index changes in 11, 5, 5, 4, and 10 residues, respectively; T143 M shows the most significant changes in the antigenic profile of HBsAg between residues 138 to 148.

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ISSN: 1743-422X

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