Baculovirus immediately early 1, a mediator for homologous regions enhancer function in trans

Table 2 Transactivation effects of hr3 enhancer on target promoters via BmNPV infection in Bm-N cells

Reporter plasmids	pSK-hr3	BmNPV	CPM	Fold-stimulation
pKS-hel510-luc	-	-	22.3 ± 4.5	1.0
	+	-	17 ± 3.8	0.8
	-	+	80 ± 13.3	3.6
	+	+	1303384 ± 74692	58447.7
pGEM3Z-lsp-luc	-	-	8 ± 2.7	1.0
	+	-	11.6 ± 4.1	1.5
	-	+	68 ± 21.2	8.5
	+	+	227636 ± 37514	28454.5

Luciferase activity indicated as CPM in 15 sec (minus background counts of cells). Transactivation of hr3 enhancer with or without virus infection presented as stimulating fold over each corresponding reporter plasmid transfection alone, that was arbitrarily set as 1.0. Each reaction contained 10 μg of protein extracted from the transfected cells.

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