Figure 1

Transactivation effects of hr3 enhancer sub-regions on different promoters via virus infection. Reporter plasmids containing different promoters were co-transfected with the hr derivates by virus infection. 10 μg of protein was extracted from the transfected cells to determine the luciferase activity. hr114 is a plasmid containing a fragment without an intact 30-bp incomplete palindrome but half of the palindrome on both sides; hr198 contains one 30-bp incomplete palindrome; hr3 contains an intact 651-bp hr3 fragment with 3 palindromes. CK had no hr added.