Figure 1
From: Construction of Metabolically Biotinylated Adenovirus with Deleted Fiber Knob as Targeting Vector
Structure, fiber trimerization and biotinylation of new 9RPSTCD adenoviral vector. (A) Structure of adenoviral vectors. All the viral vectors were replication-incompetent, E1,E3-deleted vector with a GFP as reporter gene, but each vector containing different fiber genes. The Ad.Control-GFP vector had a wild type fiber; the Ad.9R-GFP had a 9R modified fiber; the Ad.9RPSTCD-GFP had a 9R fused with PSTCD fiber. (B) Detection of biotinylation status of different viral vectors. Equal amount of purified viral vectors were loaded on SDS-PAGE gradient gels either boiled (B, to detect monomeric fibers) or unboiled (U, to detect trimeric fibers). The fiber protein and biotinylation were analyzed by western blotting using antibody against fiber protein or streptavidin-HRP. All of the viral fiber proteins formed trimers but only the virus with the PSTCD gene was biotinylated.