Figure 6

Membrane fusion activity of the truncation mutants of the F glycoprotein. The panels of F glycoprotein truncation mutants were assayed for their ability to mediate cell-cell fusion when co-expressed with their partner G glycoprotein in a quantitative vaccinia virus-based cell-cell fusion assays. Each F glycoprotein mutant was tested in triplicate wells in three independent experiments. Shown are the results of a representative cell-fusion assay with the F truncation mutants FΔCt1 through FΔCt7 along with wild-type NiV or HeV F as positive controls and vector only (pCAGGs) or media only as negative controls. (A) NiV G along with the panel of truncation mutants of NiV F. (B) HeV G along with various truncation mutants of HeV F. Error bars indicate the standard error of the mean from triplicate wells.