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Table 2 Sensitivity of the real-time RT-PCR assay and virus isolation

From: A duplex real-time reverse transcriptase polymerase chain reaction assay for detecting western equine and eastern equine encephalitis viruses

Virus

Dilution

Virus isolation

Real time RT-PCR

CT

WEEV

10-1

4/4

+

15.25

 

10-2

4/4

+

18.29

 

10-3

4/4

+

22.02

 

10-4

4/4

+

25.49

 

10-5

4/4

+

29.28

 

10-6

2/4

+

32.79

 

10-7

0/4

+

36.03

 

10-8

0/4

-

 

TCID50: 106/0.1 ml

EEEV

10-1

4/4

+

11.37

 

10-2

4/4

+

16.25

 

10-3

4/4

+

18.54

 

10-4

4/4

+

22.03

 

10-5

4/4

+

27.35

 

10-6

2/4

+

30.18

 

10-7

1/4

+

34.79

 

10-8

0/4

+

37.59

TCID50: 106.25/0.1 ml

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Virology Journal

ISSN: 1743-422X