Virus | Dilution | Virus isolation | Real time RT-PCR | CT |
---|---|---|---|---|
WEEV | 10-1 | 4/4 | + | 15.25 |
 | 10-2 | 4/4 | + | 18.29 |
 | 10-3 | 4/4 | + | 22.02 |
 | 10-4 | 4/4 | + | 25.49 |
 | 10-5 | 4/4 | + | 29.28 |
 | 10-6 | 2/4 | + | 32.79 |
 | 10-7 | 0/4 | + | 36.03 |
 | 10-8 | 0/4 | - |  |
TCID50: 106/0.1 ml | ||||
EEEV | 10-1 | 4/4 | + | 11.37 |
 | 10-2 | 4/4 | + | 16.25 |
 | 10-3 | 4/4 | + | 18.54 |
 | 10-4 | 4/4 | + | 22.03 |
 | 10-5 | 4/4 | + | 27.35 |
 | 10-6 | 2/4 | + | 30.18 |
 | 10-7 | 1/4 | + | 34.79 |
 | 10-8 | 0/4 | + | 37.59 |
TCID50: 106.25/0.1 ml |