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Table 2 Sensitivity of the real-time RT-PCR assay and virus isolation

From: A duplex real-time reverse transcriptase polymerase chain reaction assay for detecting western equine and eastern equine encephalitis viruses

Virus Dilution Virus isolation Real time RT-PCR CT
WEEV 10-1 4/4 + 15.25
  10-2 4/4 + 18.29
  10-3 4/4 + 22.02
  10-4 4/4 + 25.49
  10-5 4/4 + 29.28
  10-6 2/4 + 32.79
  10-7 0/4 + 36.03
  10-8 0/4 -  
TCID50: 106/0.1 ml
EEEV 10-1 4/4 + 11.37
  10-2 4/4 + 16.25
  10-3 4/4 + 18.54
  10-4 4/4 + 22.03
  10-5 4/4 + 27.35
  10-6 2/4 + 30.18
  10-7 1/4 + 34.79
  10-8 0/4 + 37.59
TCID50: 106.25/0.1 ml