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Figure 1 | Virology Journal

Figure 1

From: MicroRNA profiling of tomato leaf curl new delhi virus (tolcndv) infected tomato leaves indicates that deregulation of mir159/319 and mir172 might be linked with leaf curl disease

Figure 1

Phenotype of the tomato plants under study and the PCR based detection of viral DNA fragment in different tissues of ToLCNDV (2A+2B) agroinfected Pusa Ruby. a, Photographs of different leaves and flowers of tomato plant viz., Healthy tomato (cv Pusa Ruby) leaves (a) and flowers (d); ToLCNDV (2A+2B) infected tomato (cv Pusa Ruby) leaves exhibiting upward curling (b) and flowers (e); and Tomato accession LA1777 leaves (c) and flowers (f). b, Agarose gel showing amplification of Coat Protein (CP) of ToLCNDV using gDNA isolated from leaves (lane 1), flowers (lane 2), stem (lane 5) and fruits (lane 6) tissues of tomato plants agroinfected with ToLCNDV (2A+2B) while, leaves (lane 3) and flowers (lane 4) of healthy plants were used as negative controls. Amplification of CP in the lanes reflects the presence of virus in the respective tissues. Actin gene amplification served as an internal loading control.

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Virology Journal

ISSN: 1743-422X