Figure 4
From: Characterization of human adenovirus 35 and derivation of complex vectors
Determination of pIX function. (A) Relative abundance of pIX in viral particles. Approximately 1 × 1011 particles of wild type Ad35 or Ad35 with the E1 deletion d2 were fractioned as in Figure 1A and collected for subsequent mass spectroscopy analysis. The analysis of the fraction corresponding to the peak denoted 'IX' in Figure 1A is shown here with the viral sources (Ad35 wt and rAd35E1(d2) indicated. The peak at 14.1 kDa/z is the singly charged protein pIX (1+) and the peak at 7 kDa/z is the doubly charged protein pIX (2+). The abundance of pIX was normalized to hexon abundance because the two proteins co-eluted. Based on intensity the rAd35E1(d2) virus contains ~10% of wild type pIX Ad35 levels. (B) Effect of pIX on heat stability of Ad35 virions. Virions were heat treated in triplicate at 48 C for the time indicated and activity determined by an FFU assay.