Figure 3

E1B and pIX transcription mapping. (A) Schematic of E1b and pIX sequences, splice junctions shown as ^ with their coordinates that were identified by cDNA analysis, and probe locations (not to scale). Base pair coordinates of the probes are: 1 = 1641-1903, 2 = 2527-3046, 3 = 3283-3359, 4 = 3511-3853. Solid thick lines depict transcripts a, b and c as determined from cDNA and northern blot analysis (panel B) with apparent sizes given to the right in nucleotides (nt). The predicted proteins (boxes) are labeled with the name of their Ad5 homologues pIX, E1b19K, E1b55K [6], and E1b15K [11]. (B) Northern blot analysis of pIX transcripts in cells infected with wild type Ad35. Probes 1, 2, 3, 4 from panel A; a, b, c, = RNAs corresponding to putative mRNAs. (C) Schematic of Ad35 E1 region deletions with Ad35 coordinates corresponding to deletion junctions shown above each line. The viral left ITR, E1A TATAA box, and E1A, E1B, and pIX coding sequences are represented. (D) and (E) Northern blot analysis of transcripts in cells infected with wild type Ad35 (wt) or Ad35 viruses with E1 deletions and hybridized to probe 4. Location of transcripts a, b and c are indicated to the left of each blot.