Confirmation of purified GST-ORF2-E protein by SDS-PAGE and western-blotting. (A) SDS-PAGE of purified protein after elution. Lane 1: The first elution; Lane 2: The second elution; Lane 3: The third elution. (B) Western-blotting with GST monoclonal antibody. Lane 1, BL21 cell lysate before induction of IPTG; Lane 2, BL21 cell lysate after induction of IPTG; Lane 3, Purified protein. (C) and (D) are results of western-blotting using positive (C) or negative (D) porcine serum as the primary antibody. Protein marker includes 8 bands at 175, 83, 62, 47.5, 32.5, 25, 16.5, and 6.5 kDa.