Figure 3
From: Anti-HIV-1 activity of salivary MUC5B and MUC7 mucins from HIV patients with different CD4 counts
Gradient gel analysis of salivary MUC5B and MUC7 mucins from HIV negative and positive individuals. Freeze-dried samples (30 μg) of MUC5B from HIV negative individual (lane 1), MUC5B from HIV positive individuals with CD4 counts < 200 (lane 2), 200-400 (lane 3), > 400 (lane 4), MUC7 from HIV negative individual (lane 5), MUC7 from HIV positive individuals with CD4 counts < 200 (lane 6), 200-400 (lane 7) and > 400 (lane 8) were prepared in a gel loading buffer and separated in a 4-20% gradient gel. Following electrophoresis the gel was stained with PAS. While arrows in red indicates the two glycoforms of MUC7 on top of the running gel (MUC7a) and slightly entering the running gel (MUC7b), the arrow in black is at the start of running gel.