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Figure 1 | Virology Journal

Figure 1

From: Development and evaluation of an immunochromatographic strip test based on the recombinant UL51 protein for detecting antibody against duck enteritis virus

Figure 1

SDS-PAGE and western blotting analysis of recombinant UL51 protein. (a) SDS-PAGE analysis of recombinant UL51 protein. Lane M, molecular mass markers (in kDa); lane 1, extract from I mL fermentation cultures of E. coli BL21 (DE3) containing pET28a-UL51 recombinant plasmid; lane 2, the purified recombinant UL51 protein by washing inclusion bodies thrice; lane 3, refolding of inclusion bodies of the purified recombinant UL51 protein by dialyzing. (b) Western blotting analysis of recombinant UL51 protein. Lane 1, western blotting of the purified recombinant UL51 protein, with rabbit anti-DEV antibody and horseradish peroxidase (HRP)-labeled sheep anti-rabbit IgG as the first and second antibody, respectively. The arrowhead indicates the position of recombinant UL51 protein (about 34 kDa).

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Virology Journal

ISSN: 1743-422X