Time-course activation by TNFα and TSA alone or in combination with Aza-CdR in J-Lat 6.3 cells. A. GFP positive cell quantification by FACS analysis at 24, 48 and 72 h post-induction for all treatments and treatment combinations using J-Lat 6.3 cell line. GFP positive cells from the live population, defined by forward versus side scatter gating, were quantified. 20.000 events per treatment condition were analyzed. B. p24 antigen production determined using an enzyme-linked immunosorbent assay (ELISA) (Perkin Elmer, Waltham, MA) for all post-induction time points and treatments as for panel A. C. Cell viability of the different treatments and treatment combinations for all time points determined with MTS assay (Promega, Madison, WI). Final activator compound concentrations were, for Aza-CdR (2.5 μM), TNFα (10 ng/ml) and TSA (1.5 μM). Results are the mean ± standard deviation (SD).