Genetic modification of Bluetongue virus by uptake of

Table 1 Characterization of reassortant viruses.

virus	Genotyping on S7 amplicon^a	Genotyping on S10 amplicon^b	BTV6 serotype specific PCR^c	BTV8 serotype specific PCR^d
BTV8/net06	8	8	-	+
BTV6/net08	6	6	+	-
BTV6/net08/S7⁸	8	6	+	-
BTV6/net08/S10⁸	6	8	+	-

a. S7 amplicons were digested with BglII and PstI and compared to that of the parental strains, see also figure 2, lanes 8 and 9. b. S10 amplicons were sequenced and compared to sequences of parental strains BTV8/net06 and BTV6/net08. Genetic serotyping by serotype-specific real-time PCR-assays was performed for serotypes 6 (c) and 8 (d). Presence or absence of a Cp-value was interpreted as + and -, respectively.

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