Figure 2From: Viral replication is enhanced by an HIV-1 intersubtype recombination-derived Vpu proteinVpu expression was assessed by specific RT-PCR of vpu mRNA after transfection of HeLa cells. Both untransfected (UT) cells and cells transfected with empty vector (NC) were used as control (right panel). Amplification of β-actin mRNA was also evaluated as an internal control (left panel).Back to article page