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Figure 2 | Virology Journal

Figure 2

From: Identification and characterization of a virus-specific continuous B-cell epitope on the PrM/M protein of Japanese Encephalitis Virus: potential application in the detection of antibodies to distinguish Japanese Encephalitis Virus infection from West Nile Virus and Dengue Virus infections

Figure 2

Identification of the antigenic determinants on the prM/M protein with JEV-positive swine sera. (A) ELISA analysis of the short fusion peptides. Microtiter plates were coated with purified recombinant fusion protein samples (2 μg/100 μl per well). The plate was blocked with skim milk and pooled JEV-positive swine sera (dilution, 1:200) was added, following which HRP-coupled goat anti-pig IgG secondary antibody was added. Only peptide M14 showed strong reactivity with the JEV-positive swine sera. (B) Western blot analysis confirmed the result of ELISA. JEV-positive swine sera could react with GST-M14 but not with other peptide fusion protein and GST.

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Virology Journal

ISSN: 1743-422X