Figure 1
From: Integration of bovine herpesvirus 4 genome into cultured persistently infected host cell genome
Representative images of drug selected BoHV-4 persistently infected RD-4 (A) and BOMAC (B) cells, analyzed by FISH. An example of BoHV-4 hybridization signals of symmetrically labelled sister chromatids in the same spread metaphase chromosome is indicated by a white square and enlarged as an insert picture at the corner of each image. Spread chromosomes were counterstained with DAPI. Red dot signals asymmetrically labelling the chromosomes correspond to episomally-maintained BoHV-4 genomes. Green signals show hybridization of species-specific BACs. The human BAC RP11-153M12 maps on HSA 20 at 20p13 and the bovine BAC INRA-115C10 maps on BTA 4 at a subcentromeric position.