Figure 1

HIV-1 production and exosome biogenesis in Jurkat cells. A. Jurkat cells were infected with HIV-1 HXB2 viruses (HIV) or mock infected (CM). Cells (c) were harvested and culture supernatants (sp) were collected 9 days after infection. Culture supernatants were first cleared of cells and debris by low-speed centrifugation, followed by filtration and further 20% sucrose sedimentation. The virion preparations from these three steps were C, F, and S, respectively. Cell lysates and virion preparations were subjected to Western blotting using antibodies against HIV-1 p24 or β-actin (upper: sucrose banding for 1 hr; lower: sucrose banding for 2.5 hr). *: p24 precursors. B. HIV-1 RT assay of three virion preparations. C. Acetylcholinesterase (AChe) activity assay of the virus preparations F and S as well as the sucrose cushion from sucrose sedimentation (S*). D. Jurkat cells were inoculated with each of three virus preparations (corresponding to 10,000 cpm of RT activity) and monitored for virus infection and replication.