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Table 5 Yields Obtained with H5N1 Reverse Genetics Constructs in an A/PR/8/1934 Vaccine Strain Background Grown in Bagged ECE.

From: Gas-permeable ethylene bags for the small scale cultivation of highly pathogenic avian influenza H5N1 and other viruses in embryonated chicken eggs

Strain Log10TCID50/mla
A/Anhui/01/2005 (H5N1)-PR8-IBCDC-RG 8.7
A/VNH5N1-PR8/CDC-RG 10.0
  1. aTCID50/ml of CAS fluids in MDCK cells in serum-free growth medium plus trypsin. CAS fluids were pooled from four ECE and harvested 48 - 72 hrs after infection of 9-day old ECE with 102 to 103 TCID50 units of virus and incubation at 34°C.