Figure 3

Molecular Clock of DENV-1. DENV-1 divergence time was estimated using year of isolation (scale used in the tree) as calibration points under the strict molecular clock model using GTR+Γ+I parameters. Posterior Probability (PP) values are indicated for each node, and the extent of the 95% highest posterior density (HPD) intervals for each divergence time is represented by the blue bars. Branches representing America genotype including Colombian isolates were collapsed (presented in red) for clearness purposes. Root of the tree was previously calculated by TRACE statistic interface of BEAST, and is placed in 1924 as the most recent ancestor