Figure 3From: Amino acid substitutions in the E2 glycoprotein of Sindbis-like virus XJ-160 confer the ability to undergo heparan sulfate-dependent infection of mouse embryonic fibroblastsNeurovirulence of the viruses for suckling mice. Suckling mice (three days old) were inoculated intracerebrally with 30 μl of 103 PFU/ml recombinant or parental viruses; equal volume of Eagle's fortified with 1% fetal bovine serum was used as control.Back to article page