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Table 2 Optimization of CIC concentration

From: Failure to detect Xenotropic murine leukaemia virus-related virus in Chinese patients with chronic fatigue syndrome

(1) Multiplex real-time PCR
pACYC-MS2- IC-2V plasmid CIC concentration (copies/μl) pACYC-MS2-2V plasmid standard 1 × 105copies/μl pACYC-MS2-2V plasmid standard 1 × 104copies/μl pACYC-MS2-2V plasmid standard 1 × 103copies/μl pACYC-MS2-2V plasmid standard 1 × 102copies/μl XMRV 0 copies/μl
  CIC (HEX)Ct XMRV (FAM)Ct CIC (HEX)Ct XMRV (FAM)Ct CIC (HEX)Ct XMRV (FAM)Ct CIC (HEX)Ct XMRV ((FAM)Ct CIC (HEX)Ct
100000 39.22 28.79 32.18 31.58 28.92 34.81 28.50 No Ct 28.34
10000 37.85 28.12 31.26 31.06 32.36 34.56 31.45 44.26 31.55
1000 39.52 24.73 37.20 27.76 31.50 31.18 35.79 34.28 34.11
0 No Ct 24.69 No Ct 28.47 No Ct 30.73 No Ct 33.81 No Ct
(2) Multiplex real-time RT-PCR
Armored RNA concentration (IU/ml) Armored RNA standard 1 × 10 5 IU/ml Armored RNA standard 1 × 10 4 IU/ml Armored RNA standard 1 × 10 3 IU/ml Armored RNA standard 1 × 10 2 IU/ml XMRV 0 IU/ml
  CIC (HEX)Ct XMRV (FAM)Ct CIC (HEX)Ct XMRV (FAM)Ct CIC (HEX)Ct XMRV (FAM)Ct CIC (HEX)Ct XMRV (FAM)Ct CIC (HEX)Ct
100000 39.50 25.24 30.89 28.28 27.12 No Ct 26.35 No Ct 26.07
10000 37.96 25.00 30.70 28.22 30.37 31.17 27.79 37.06 29.49
1000 No Ct 25.25 30. 65 28.03 33.18 31.14 33.87 34.40 33.27
0 No Ct 25.18 No Ct 27. 82 No Ct 30.78 No Ct 37.11 No Ct
  1. Ct values indicate the standard concentrations. A sample with Ct > 45 cycles was considered to be negative. Concentrations of XMRV DNA/RNA and pACYC-MS2-2V plasmid/armored RNA standard were indicated by FAM and HEX signals, respectively.