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Figure 3 | Virology Journal

Figure 3

From: Substitution of adeno-associated virus Rep protein binding and nicking sites with human Chromosome 19 sequences

Figure 3

Viral DNA replication. (A) Restriction map of pSub201(-). (B) Location and orientation of the AAV2 genome in pSub201(-). (C) Southern blot of genomic DNA from cells cotransfected with an adenovirus helper plasmid and the indicated AAV2 plasmids. The membrane was probed with random primer 32P-labeled, linearized pSub201(-). The molecular weight marker is homologous to the backbone portion of pSub201. (D) Southern blot of genomic DNA from cells cotransfected with an adenovirus helper plasmid and pVM108. The membrane was probed with random primer labeled pSub201. The pSub201(-) marker was made by running XbaI and PvuII digests in the same lane.

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Virology Journal

ISSN: 1743-422X