Figure 1
From: Evaluation of adenovirus capsid labeling versus transgene expression
Analysis of in vitro DNA replication of Ad-wt-pIX-Luc and Ad-wt-E3-Luc. A549 cells were infected at an MOI of 10 IP/cell of Ad-wt-pIX-Luc, Ad-wt-E3-Luc, Adwt, or non-replicative virus. On days 0, 2, 4, and 6 post-infection media from infected cells were collected along with total DNA from infected cells. DNA was extracted from media and cell lysate was isolated from cells according to a standard protocol, using DNeasy tissue kit. In brief, E4 copy number was determined by Real-Time quantitative PCR. The E4 copies were normalized against human DNA concentration. These data samples were analyzed using a LightCycler 480, software 1.5.0SP1. The values are expressed as the mean ± standard deviation of three replicates. The asterisk (*) indicates a P value = 0.018. The asterisks (**) indicates a P value = 0.002. Comparisons were performed using analysis of variance followed by the Tukey-Kramer multiple comparisons test.