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Table 1 Primers used for amplification of the complete genomic sequence of FMDV O/Fujian/CHA/5/99 strain[a]

From: Genetic characterization of the cell-adapted PanAsia strain of foot-and-mouth disease virus O/Fujian/CHA/5/99 isolated from swine

Primers

Nucleotide sequence (5'-3')

Position

S+

TTGAAAGGGGGCGCTAGGGTCT

1-22

Pan/S-

AAAACTTAGGGGGGGGGGGGGGGGGGGGTGAAAGG

362-376[b]

Pan/I+

CCTTTCACCCCCCCCCCCCCCCCCCCCTAAGTTTT

362-376[b]

L3

GTTCTGGTACTGCTGCATGTAG

1759-1780

Pan/204

ACCTCCAACGGGTGGTACGC

1544-1563

NK61

GACATGTCCTCCTGCATCTG

3998-4017

P211

CGCTGCCTACCTCCTTCAAT

3726-2745

P222

ACTATCTCAAAGTTTTCCTTCAG

5519-5541

Pan/201

ACGAGAAGGTGTCGAGCCACC

5322-5342

Pan/205

TGTACGCGCTCCTCAACATCTC

6687-6708

D3+

CAAGGCGGGTTACTGTGGAGGAG

6502-6524

Dnn-

GCGGCCGCCATATGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT

3' end

  1. [a] The primer pairs used in this study were designated based on the FMDV O/Tibet/CHA/1/99 strain [19].
  2. [b] The primers position was calculated without 20 G/C.
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Virology Journal

ISSN: 1743-422X