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Figure 2 | Virology Journal

Figure 2

From: A single dose of DNA vaccine based on conserved H5N1 subtype proteins provides protection against lethal H5N1 challenge in mice pre-exposed to H1N1 influenza virus

Figure 2

IFN-γ secreting splenocytes in unexposed and pre-exposed mice after immunization. BALB/c mice were randomized into two groups, one infected with H1N1 virus and the other uninfected. Six weeks later, both groups were divided into 4 subgroups. Three of the subgroups were immunized with H5 NP DNA, M1 DNA or NP + M1 DNAs, respectively, and the rest subgroup remained unimmunized. Splenocytes of mice were isolated 6 weeks after immunization and stimulated by the synthesized NP or M1 peptide. The number of IFN-γ secreting splenocytes was calculated as the average number of spots in the triplicate stimulant wells. Abscissa description: NP + M1 (NP): immunization with NP + M1 DNAs and stimulation with NP peptide; NP + M1 (M1): immunization with NP + M1 DNAs and stimulation with M1 peptide; NP: immunization with NP DNA and stimulation with NP peptide; M1: immunization with M1 DNA and stimulation with M1 peptide; C (NP): unimmunization control and stimulation with NP peptide; C (M1): unimmunization control and stimulation with M1 peptide. a Significant difference (p < 0.05), compared with the corresponding unexposed mice. b Significant difference (p < 0.05), compared with the pre-exposed but unimmunized control.

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Virology Journal

ISSN: 1743-422X