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Figure 3 | Virology Journal

Figure 3

From: A 176 amino acid polypeptide derived from the mumps virus HN ectodomain shows immunological and biological properties similar to the HN protein

Figure 3

Hemadsorption and neuraminidase activities in the pcDNAHN176 transfected cells. A). Hemadsorption (HD) reaction. a) Mock infected cells; b) MuV-infected cells; c) pcDNA-3.1-transfected cells; d) pcDNAHN176-transfected Vero cells. The red cell aggregates indicate a positive HD. 40×. B) Neuraminidase (NA) reaction in total cellular protein extract by a dot blot assay: a) Mock infected cells; b) MuV-infected cells; c) pcDNA-3.1-transfected cells; d) pcDNAHN176-transfected Vero cells. C) % of hemoglobin (Hb) released after the HD. The Hb absorbance of MuV-infected cells was considered as 100% and was used to calculate the % of Hb for the different cells. D) Comparison of the NA activity in MuV-infected cells, pcDNA3.1-transfected cells and pcDNAHN176-transfected Vero cells by dot blot and spectrophotometric methods.

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Virology Journal

ISSN: 1743-422X