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Table 6 Real time PCR assays for VHS virus, Paranucleospora theridion, Flavobacterium psychrophilum, Candidatus Piscichlamydia salmonis and a new species of chlamydia from gills of Atlantic salmon.

From: Characterization of a VHS virus genotype III isolated from rainbow trout (Oncorhychus mykiss) at a marine site on the west coast of Norway

Target Code: Primers/probe Amplicon size Reference
VHS virus VHSV F08 TGT CCG TKC TTC TCT CCT ATG TAC T   Modified
  VHSV probe CTC ACA GAC ATG GG 109 nt [44]
  VHSV R08 GCC CTG RCT GMC TGT GTC A   Modified
Paranucleospora PT-F CGG ACA GGG AGC ATG GTA TAG   
theridion PT-probe TTG GCG AAG AAT GAA A 59 nt [49]
  PT-R GGT CCA GGT TGG GTC TTG AG   
Flavobacterium Flavo-R TGT AAA CTG CTT TTG CAC AGG AA   
psychrophilum Flavo-probe AAA CAC TCG GTC GTG ACC 72 nt Present study
  Flavo-F GAT CCT TAT TCT CAC AGT ACC GTC AA   
Candidatus Pch-F TCA CCC CCA GGC TGC TT   
Piscichlamydia Pch-probe CAA AAC TGC TAG ACT AGA GT 60 nt Present study
salmonis Pch-R GAA TTC CAT TTC CCC CTC TTG   
New species of Sch-F GGG TAG CCC GAT ATC TTCA AAG T   
gill chlamydia Sch-probe TCC TTC GGG ACC TTA C 66 nt Present study
  Sch-R CCC ATG AGC CGC TCT CTC T   
Elongation factor EL1A-elaf CCC CTC CAG GAC GTT TAC AAA   
1 alpha EL1A-elam1 ATC GGT GGT ATT GGA AC 57 nt [45]
S. salar EL1A-elar CAC ACG GCC CAC AGG TAC A