N-N interaction of truncated UUKV-N protein constructs in the M2H assay and verification of the protein expression.(A) Truncation ΔN19 affected the N-N interaction, ΔN34 and C-terminal truncation ΔC10 destroyed the oligomerization ability completely. Deleted regions are shown in white. Numbers are averages of normalized luciferase activity values (%), where the wt N-N interaction was set as 100%. ± standard deviations are calculated for the mean values. (B) N protein expression of the M2H constructs was verified in immunoblotting. MAbs were used to detect the N protein fusions with DNA-AD (lanes 1 to 4), and DNA-BD (lanes 5 to 8) constructs, which migrated as 44-46 kDa bands.